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60kD熱休克蛋白(Hsp60)多克隆抗體

DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 10μg/ml Rabbit Anti-Mouse Hsp60 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: Mouse Heart lysate Primary Ab: 0.2μg/ml Rabbit Anti-Mouse Hsp60 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: NIH/3T3 cell lysate; Lane2: MCF7 cell lysate;
Primary Ab: 0.2μg/ml Rabbit Anti-Mouse Hsp60 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對(duì)Hsp60的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別Hsp60。

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

雜志	參考文獻(xiàn)
Sci Rep	Hsp60 response in experimental and human temporal lobe epilepsy [PubMed: 25801186]
Cellular?and Molecular Gastroenterology and Hepatology	Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro [Pubmed: 30878663]
Breast Cancer Res Treat	Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer [Pubmed: 32601970]

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編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA822Mu01	60kD熱休克蛋白(Hsp60)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA822Mu02	60kD熱休克蛋白(Hsp60)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA822Mu01	60kD熱休克蛋白(Hsp60)多克隆抗體	WB; IHC; ICC/IF
UPAA822Mu02	60kD熱休克蛋白(Hsp60)多克隆抗體	WB; IHC; ICC/IF
USEA822Mu	60kD熱休克蛋白(Hsp60)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA822Mu	60kD熱休克蛋白(Hsp60)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA822Mu	60kD熱休克蛋白(Hsp60)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA822Mu01	60kD熱休克蛋白(Hsp60)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".