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微管蛋白β(TUBb)單克隆抗體

Monoclonal Antibody to Tubulin Beta (TUBb)

TUB-B; TUBB5; TUBB1; M40

編號UMAB870Hu22
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)C1
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB870Hu01-微管蛋白β(TUBb)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Cavia (Guinea pig ，豚鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 389 907 ¥ 907 ¥ 1296 ¥ 3240 ¥ 12960
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)

WB圖
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Cavia Kidney lysate; Lane4: Canine Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Kidney lysate; Lane7: Rabbit Cerebrum lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Human SH-SY5Y cell line was stained with 10μg/ml mouse Anti-human TUBb Monoclonal Antibody (Catalog MAB870Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對TUBb的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別TUBb。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Flow cytometry: 10μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(xué)(IHC)實驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實驗服務(wù)
流式細(xì)胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實時熒光定量PCR實驗服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
archives of pharmacal research	Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473]
archiv der pharmazie (Weinheim).	New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327]
ACS?Applied?Materials?&?Interfaces	Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513]
Future?Medicinal Chemistry	Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents [Pubmed:29779400]
Future?Medicinal Chemistry	Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization [Pubmed:29966433]
Journal of Biological Chemistry	Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167]
Journal?of?Enzyme?Inhibition?and?Medicinal?Chemistry	Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors [Pubmed: 30191744]
European Journal of Medicinal Chemistry	Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study [Pubmed: 31536891]
ACS Sensors	Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform [Pubmed: 32207606]
Bioorganic Chemistry	Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their?… [Pubmed: 32325332]
BRITISH JOURNAL OF NUTRITION	Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437]
European Journal of Medicinal Chemistry	Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon?… [Pubmed: 33158579]
J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS	Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells [33300466]
Cancer Cell International	Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int	Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Sci Rep	Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026]

留言咨詢

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UAPB870Hu01	微管蛋白β(TUBb)活性蛋白	Cell?culture;?Activity?Assays.
URPB870Hu01	微管蛋白β(TUBb)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UCAB870Hu01	抗微管蛋白β(TUBb)多克隆抗體	WB
UPAB870Hu01	微管蛋白β(TUBb)多克隆抗體	WB; IHC; ICC/IF
UMAB870Hu22	微管蛋白β(TUBb)單克隆抗體	WB; IHC; ICC/IF; FCM
UMAB870Hu23	微管蛋白β(TUBb)單克隆抗體	WB; IHC; ICC/IF
UMAB870Hu25	微管蛋白β(TUBb)單克隆抗體	WB; ICC/IF
UMAB870Hu27	微管蛋白β(TUBb)單克隆抗體	WB; ICC/IF
UMAB870Hu24	微管蛋白β(TUBb)單克隆抗體	IHC
UMAB870Hu21	微管蛋白β(TUBb)單克隆抗體	WB; IHC; ICC/IF
UCAB870Hu22	抗微管蛋白β(TUBb)單克隆抗體	WB; IHC; ICC/IF
UFAB870Hu06	抗微管蛋白β(TUBb)單克隆抗體	FCM
UFAB870Hu04	抗微管蛋白β(TUBb)單克隆抗體	FCM
UFAB870Hu02	抗微管蛋白β(TUBb)單克隆抗體	FCM
UFAB870Hu08	抗微管蛋白β(TUBb)單克隆抗體	FCM