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血管生成素樣蛋白4(ANGPTL4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Angiopoietin Like Protein 4 (ANGPTL4)

ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein

  • 血管生成素樣蛋白4(ANGPTL4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血管生成素樣蛋白4(ANGPTL4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)血管生成素樣蛋白4(ANGPTL4)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
URPB019Hu02 血管生成素樣蛋白4(ANGPTL4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB019Hu01 血管生成素樣蛋白4(ANGPTL4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAB019Hu01 血管生成素樣蛋白4(ANGPTL4)多克隆抗體 WB; IHC
UMAB019Hu22 血管生成素樣蛋白4(ANGPTL4)單克隆抗體 WB; IHC; ICC; IP.
USEB019Hu 血管生成素樣蛋白4(ANGPTL4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB019Hu 血管生成素樣蛋白4(ANGPTL4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSB019Hu01 血管生成素樣蛋白4(ANGPTL4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
The Journal of Biological Chemistry Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes [PubMed: PMC3318686]
J Clin Endocrinol Metab. Decreased Concentrations of the Lipoprotein Lipase Inhibitor Angiopoietin-Like Protein 4 and Increased Serum Triacylglycerol Are Associated With Increased Neonatal Fat Mass in Pregnant Women With Gestational Diabetes Mellitus [Pubmed: 23744407]
PloS one Lack of Relationship between Cord Serum Angiopoietin-Like Protein 4 (ANGPTL4) and Lipolytic Activity in Human Neonates Born by Spontaneous Delivery [Pubmed: 24324678]
Journal of Hematology & Oncology Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy [PubMed: 26054961]
Gynecol Endocrinol Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome [PubMed: 26291814]
Molecular Nutrition & Food research Interaction of Gut Microbiota and High‐sodium, Low‐potassium Diet in Altering Plasma Triglyceride Profiles Revealed by Lipidomics Analysis [Pubmed: 31675161]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
Food Chem Toxicol Di (2-ethylhexyl) phthalate exposure exacerbates metabolic disorders in diet-induced obese mice [34303773]
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