血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)
VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein
- 編號UKSA145Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1000pg/mL
- 可適靈敏度6.6pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3283 ¥ 5472 ¥ 9850 ¥ 19152 ¥ 32832
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對血管內(nèi)皮生長因子C(VEGFC)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA145Ra01 | 血管內(nèi)皮生長因子C(VEGFC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA145Ra01 | 血管內(nèi)皮生長因子C(VEGFC)多克隆抗體 | WB |
| UFAA145Ra01 | 抗血管內(nèi)皮生長因子C(VEGFC)多克隆抗體 | FCM |
| ULAA145Ra81 | 血管內(nèi)皮生長因子C(VEGFC)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA145Ra71 | 血管內(nèi)皮生長因子C(VEGFC)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA145Ra22 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | WB |
| UMAA145Ra21 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | WB |
| UFAA145Ra02 | 抗血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | FCM |
| ULAA145Ra72 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA145Ra | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA145Ra | 血管內(nèi)皮生長因子C(VEGFC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA145Ra01 | 血管內(nèi)皮生長因子C(VEGFC)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA145Ra01 | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Cellular Biochemistry | Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma [PubMed: 19241443] |
| genetics and molecular research | Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages [PubMed: 26125824] |
| Experimental and Therapeutic | Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia [PubMed: 26622498] |
| Am J Pathol | IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages [PubMed: 26608451] |
| PLoS One. | Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. [pubmed:27383632] |
| Oncology?Letters | RNAi-mediated?gene?silencing?of?vascular?endothelial?growth?factor?C?suppresses?growth?andinduces?apoptosis?in?mouse?breast?cancer?in?vitro?and in?vivo. [pubmed:27895746] |
| Angiogenesis | Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering [Pubmed:29582235] |
| Oncology Reports | Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells [Pubmed:29328465] |
| LABORATORY INVESTIGATION | Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease [Pubmed: 31019289] |
| Cutaneous and Ocular Toxicology | Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions? [Pubmed: 32722957] |
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