冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)活性蛋白
Active Cold Inducible RNA Binding Protein (CIRBP)
CIRP; A18HNRNP; Glycine-Rich RNA Binding Protein
- 編號UAPG886Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點9.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPG886Hu01 | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPG886Hu01 | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAG886Hu01 | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)多克隆抗體 | WB; IHC; ICC/IF |
| UMAG886Hu21 | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)單克隆抗體 | WB; IHC; ICC; IP. |
| USEG886Hu | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMG886Hu | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UCGG886Hu01 | 冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| J Cell Mol Med | Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission [34786826] |
