核糖核酸酶A(RNase A)活性蛋白
Active Ribonuclease A (RNase A)
RNASE1; RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease,RNase A Family,1(Pancreatic)
- 編號(hào)UAPA297Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)8.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 576 ¥ 1440 ¥ 2880 ¥ 8640 ¥ 21600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Ribonuclease A (RNASEA) is a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. RNASEA cleaves RNA on the 3' side of pyrimidine nucleotides. The protein acts to degrade ds-RNA over ss-RNA. The activity of recombinant human RNASEA measured by cleaving yeast RNA. One unit of the enzyme causes an increase in absorbance of 0.001 at 260 nm in 15 min when yeast RNA is hydrolyzed at 50°C and pH 5.0. Pipette 50ulof respective recombinant human RNASEA dilution into 100ul 0.1M sodium acetate buffer, pH 5.0, then add 150ul of 0.15mg/ml yeast RNA. The blank tube use 50ul ultrapure water instead of enzyme dilution. All the all tubes Incubate at 50°C for 15 minutes. Read A260 versus blank.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA297Hu01 | 核糖核酸酶A(RNase A)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA297Hu01 | 核糖核酸酶A(RNase A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA297Hu02 | 核糖核酸酶A(RNase A)多克隆抗體 | WB; IHC |
| UPAA297Hu01 | 核糖核酸酶A(RNase A)多克隆抗體 | WB; IHC |
| ULAA297Hu71 | 核糖核酸酶A(RNase A)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA297Hu22 | 核糖核酸酶A(RNase A)單克隆抗體 | WB; IHC; ICC; IP. |
| UMEA297Hu | 核糖核酸酶A(RNase A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA297Hu | 核糖核酸酶A(RNase A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA297Hu | 核糖核酸酶A(RNase A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Molecular Sciences | The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study [Pubmed:26927088] |
| Investigative Ophthalmology & Visual?Science | The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease [Pubmed:30025106] |
| Nat Commun | Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation [33986289] |
