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谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體

Polyclonal Antibody to Transglutaminase 2 (TGM2)

tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H

感染免疫
基因敲除驗證

編號UPAB830Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB830Hu01-谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
DAB staining on IHC-P; Sample: Human Uterus Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P; Sample: Human Lymph node Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P; Sample: Human Uterus Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Western Blot; Sample: Hela cell lysate Primary Ab: 0.3μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: TGM2 knockout Hela cell lysate;
Predicted MW: 77kd
Observed MW: 77kd
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Placenta lysate; Lane3: HepG2 cell lysate; Lane4: K562 cell lysate; Lane5: Hela cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對TGM2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別TGM2。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(xué)(IHC)實驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實驗服務(wù)
流式細(xì)胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實時熒光定量PCR實驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB830Hu01	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPB830Hu01	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)活性蛋白	Cell?culture;?Activity?Assays.
UPAB830Hu01	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體	WB; IHC; ICC/IF
ULAB830Hu71	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體(生物素標(biāo)記)	WB
UMAB830Hu22	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)單克隆抗體	WB; IHC; ICC; IP.
UMEB830Hu	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEB830Hu	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UHEB830Hu	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCB830Hu	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMB830Hu	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSB830Hu01	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".
UCGB830Hu01	谷氨酰胺轉(zhuǎn)胺酶2(TGM2)cDNA	PCR、蛋白表達(dá)等

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Connective Tissue Research	Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells [Pubmed:27115725]
American Journal of Respiratory Cell and Molecular Biology	ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. [pubmed:29053339]
3 Biotech	Metformin attenuates TGF?β1?induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF?β pathways [Pubmed: 32550106]

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