Ⅳ型膠原(COL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Collagen Type IV (COL4)
Type-IV Collagen
- 編號UKSA180Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍7.8-500ng/mL
- 可適靈敏度3.1ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對Ⅳ型膠原(COL4)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA180Hu01 | Ⅳ型膠原(COL4)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA180Hu01 | Ⅳ型膠原(COL4)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA180Hu71 | Ⅳ型膠原(COL4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA180Hu | Ⅳ型膠原(COL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA180Hu | Ⅳ型膠原(COL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA180Hu | Ⅳ型膠原(COL4)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA180Hu | Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA180Hu01 | Ⅳ型膠原(COL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| American Journal fo Reproductive Immunology | Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1 [Pubmed:24548186] |
| Chem Biol Interact | Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis [PubMed: 25617480] |
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| International Journal of Experimental Pathology | Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non-alcoholic steatohepatitis model, induced by high-fat and high-cholesterol diet [doi:10.1111] |
| MOLECULAR AND CLINICAL ONCOLOGY | Clinical significance of serum laminin and typea??IV collagen levels in cutaneous melanoma patients [Pubmed:27330797] |
| Am J Transplant. | Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. [pubmed:27456745] |
| Molecular Medicine Reports | Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte?associated immunoglobulin?like receptor 1 [10.3892:mmr.2017.6921] |
| Clinical?Cancer?Research | Phase I and biomarker study of plerixafor and bevacizumab in recurrent high-grade glioma [Pubmed:29941486] |
| International Journal of Molecular Medicine | Adenovirus?mediated knockdown of activin A receptor type 2A attenuates immune?induced hepatic fibrosis in mice and inhibits interleukin?17?induced?… [Pubmed:29620144] |
| Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities [Pubmed: 30530231] |
| Experimental Lung Research | High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects [Pubmed: 31290711] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs [Pubmed: 32146192] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| Biomolecules | Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study [Pubmed:35625637] |
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